Fig. 5.

LncRNA HIF1A-AS2 modulates the expression of ATF2 via binding to USF1. (A) Prediction of the lncRNA-TF-mRNA interaction based on the LncMAP database. (B) The protein expression of USF1 and ATF2 in ECs, SMCs and HCAECs exposed to ox-LDL measured by Western blot analysis. (C) The binding of lncRNA HIF1A-AS2 to USF1 in ECs, SMCs and HCAECs detected by RNA pull-down. (D) The binding of lncRNA HIF1A-AS2 to USF1 in ECs detected by RIP assay. (E) The binding site of USF1 on the ATF2 promoter predicted from JASPAR website. (F) The binding of USF1 to the ATF2 promoter region detected by the dual-luciferase reporter assay. (G) The binding of USF1 to the ATF2 promoter in ECs with or without silencing lncRNA HIF1A-AS2 detected by ChIP assay. *p < 0.05 vs. the untreated cells or ox-LDL-exposed cells treated with oe-NC or IgG. Data (mean ± standard deviation) were obtained from three independent experiments and those between groups were analyzed by unpaired t test.