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. 2020 Jul 31;26:123–135. doi: 10.1016/j.jare.2020.07.015

Fig. 7.

Fig. 7

Knockdown of lncRNA HIF1A-AS2 or ATF2 inhibits inflammation in atherosclerotic ApoE−/− mice in vivo. (A) Determination of lncRNA HIF1A-AS2 and ATF2 expression in mouse arterial tissues by RT-qPCR. (B) Lipid deposition in injured aorta areas observed after Oil red O staining. (C) HE staining for detection of thoracic aortic injury area (100×). (D) Lipid deposition in the thoracic aorta observed after Oil red O staining (100×). (E) ELISA detection of TNF-α, IL-1β, IL-6 expression in the serum of ApoE−/− mice fed with HFD. (F) VCAM-1, ICAM-1, and MCP-1 protein expression in mouse arterial tissues measured by Western blot analysis. *p < 0.05 vs. the ApoE−/− mice fed with HFD and injected with lentivirus vector expressing sh-NC; #p < 0.05 vs. the ApoE−/− mice fed with HFD and injected with lentivirus vector expressing sh-HIF1A-AS2. Data (mean ± standard deviation) among multiple groups were analyzed by one-way analysis of variance. n = 20 for mice in each group.