Fig. 3.

Flow cytometry analysis of the inhibitory effect of compound 7f on A549 and PC-9 cells. (A) and (B): a. PI staining showed that 7f blocks the cycle of A549 or PC-9 cells. (A) and (B): b. Annexin V-FITC/PI dual staining showed that compound 7f induced apoptosis in A549 or PC-9 cells. (A) and (B): c. JC-10 staining demonstrated the effect of compound 7f on A549 or PC-9 cell mitochondrial membrane potential. Three independent experiments were performed, each column represented the mean ± SD of triplicate determinations (where *p < 0.05, **p < 0.01 and ***p < 0.005 compared to control).