Fig. 5.

Compound 7f could inhibit angiogenesis in vitro and in vivo. (A) ELISA determined the content of VEGF in the tumor supernatant. (B) The effect of tumor supernatant on the formation of the tube of HUVECs in vitro at different drug concentrations. (C) Effect of 7f on tumor supernatant-induced angiogenesis assessed on the CAM model. The bar graphs represent the angiogenic index by counting vessel branch points using Image-Pro Plus software in a double-blinded manner. Data represent the mean of their replicates (where *p < 0.05, **p < 0.01 and ***p < 0.005 compared to control).