Fig. 1.

BT changes the levels of EMT markers. (A) The structure of BT. (B) HCCLM3 cells were exposed to BT (0, 1, 3, 5, 10, 25, 50, 100 nM) for 24 h and viability was calculated by MTT method. (C) HCCLM3 cells were exposed to BT and proliferation assay was performed using RTCA for 72 h. (D-E) HCCLM3 cells were exposed to BT for 24 h and Western blotting was executed. (F) Total RNA was measured via real-time PCR for levels of different genes. * p < 0.05; ** p < 0.01 and *** p < 0.001 as measured by (G) HCCLM3 cells were exposed to 10 nM of BT for 24 h, and then distribution of Vimentin and Occludin was studied by immunocytochemistry.