Fig. 2.

BT reduces invasion and blocks the STAT3 pathway. (A) HCCLM3 invasive activity in Matrigel-coated plate was determined. (B) HCCLM3 cells were exposed to 10 nM of BT for 8 h and invasion assay was done. (C) HCCLM3 cells were exposed to BT for 4 h and Western blot was executed. (D) HCCLM3 cells were transiently transfected with scrambled or STAT3 siRNA and then exposed to10 nM of BT for 4 h and blotting was carried out. (E-F) Transfection was done with 50 nM STAT3 siRNA or scrambled siRNA for 24 h as narrated above in D. The cells were processed as narrated in C and blotting was conducted.