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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2020 Aug 11;147:49–61. doi: 10.1016/j.yjmcc.2020.07.012

Figure 6. Altered sarcomere activation due to cardiomyopathic mutant sarcomeric protein expression in live cardiac muscle.

Figure 6.

A, Ensemble average traces of tension recordings in papillaries show restrictive cardiomyopathy cTnI mutant R193H have alterations in relaxation kinetics, ΔTension bar represents a 10% change from baseline. B, Summary statistics of papillary twitch tension shows R193H slows relaxation kinetics (n=6 for control and n=5 for R193H mutants). C, Ensemble average traces of FRET ratio recordings in papillaries show R193H mutants have slowed relaxation kinetics, ΔRatio bar represents a 10% change in FRET from baseline. D, Summary statistics of papillary FRET ratio shows R193H mutation slows relaxation kinetics (n=6 for control and n=4 for R193H mutants). A-D, Data obtained at 22°C with 1.0Hz stimulation. Mean ± S.E.M are presented. Unpaired t-tests were used, as appropriate: *P < 0.05.