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. 2020 Oct 5;117(42):26237–26244. doi: 10.1073/pnas.2004657117

Fig. 5.

Fig. 5.

sGN∆tryp dimerizes weakly in solution. (A) A top view of the noncovalent dimerization interface. In the magnified box in the Top Left is a close-up view of the S214 that was mutated to cysteine. (B) SEC elution profile of purified sGN∆tryp−S241C and the corresponding nonreducing SDS-PAGE (Coomassie brilliant blue stained) of the peak fractions. The Inset shows a 2Fobserved-Fcalculated (2Fo-Fc) electron density map obtained from the sGN∆tryp−S241C crystals, confirming the existence of the C214–C214′ disulfide bond.