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. 2020 Oct 5;117(42):26298–26306. doi: 10.1073/pnas.2016954117

Fig. 2.

Fig. 2.

[PSI+] prion variants are lost in heterozygous diploids formed with an isogenic WT strain. (Top) Original haploids of [PSI+] strains (WT MS224, [PSI+] isolates in each deletion strain MS515, MS527, and MS510: left to right), and [psi−] strains (MS327, MS520, MS528, and MS514: left to right), and Ura− segregants (Top to Bottom) were transformed with a 2-µ plasmid pH770/pM18 encoding Sup35NM-GFP controlled by the GAL1 promoter. Each Ura− segregant was obtained from a 4 Ura−: 0 Ura+ tetrad of the indicated heterozygous diploid, and its genotype was examined by replica-plating. The genotype of cells examined is shown above the image. After GAL induction for 16 h in 2% (wt/vol) raffinose, 2% (wt/vol) galactose minimal medium, Sup35NM-GFP aggregates or diffused GFP signal were observed using fluorescence confocal microscopy (magnification 1500×).