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[PSI+sbs], [PSI+zos], and [PSI+szs] prion variants are differentiated by their propagation ability in doubly heterozygous diploids. [PSI+sbs], [PSI+zos], and [PSI+szs] isolates were mated on YPAD with ssb1/2Δ [psi−] MS520, zuo1Δ [psi−] MS528, ssz1Δ [psi−] MS514, or ssb1/2Δzuo1Δ [psi−] MS560. Doubly heterozygous diploids were replica-plated to minimal media with and without uracil, supplemented with adenine and histidine for diploid selection.
