Table 1.
De novo generation of [PSI+] variants is elevated in ssb-rac deficient cells
| Total Ura+/106 cells | ||
| Host | Spontaneous [PSI+] | Induced [PSI+] |
| WT | 0.36 ± 0.1 | 43 ± 6 |
| ssb1Δssb2Δ | 7.2 ± 0.5 | 1,740 ± 200 |
| zuo1Δ | 6.0 ± 0.7 | 1,600 ± 160 |
| Ssz1Δ | 4.5 ± 0.4 | 2,200 ± 230 |
Strains MS327, MS515, MS527, and MS510 (top to bottom) carry the same [PIN+]. For spontaneous [PSI+], cells were grown 2 d in 2% glucose liquid culture at 30 °C, and 107 yeast cells were spread on standard SC plates without uracil. For induced [PSI+] formation, strains carrying p1520 with SUP35NM driven by a galactose-inducible promoter were grown 2 d in 2% galactose/2% raffinose medium at 30 °C, and 105 yeast cells were spread on SC plates without uracil. The average number of colonies formed after 5 d of incubation at 30 °C is shown (the data from at least three independent experiments were combined). Numbers represent Ura+ colonies with [PSI+] that were confirmed by GuHCl curability. Ura+ colonies ± SD is shown.