Table 2.
[PSI+] isolates are more stable in ssb-racΔ strains than in the ssb-racΔ/+ diploid strains
| Isolate | Ura+/total subclones | ||||||||
| [PSI+sbs] in | [PSI+zos] in | [PSI+szs] in | |||||||
| ssb1/2Δ | ssb1/2Δ/+ | ssb1/2Δ/− | zuo1Δ | zuo1Δ/+ | zuo1Δ/− | ssz1Δ | ssz1Δ/+ | ssz1Δ/− | |
| 1 | 30/30 | 1/20 | 20/20 | 28/30 | 3/20 | 18/20 | 30/30 | 1/20 | 18/20 |
| 2 | 30/30 | 2/20 | 20/20 | 30/30 | 0/20 | 19/20 | 30/30 | 0/20 | 19/20 |
| 3 | 25/30 | 1/20 | 20/20 | 22/30 | 1/20 | 19/20 | 29/30 | 0/20 | 20/20 |
| 4 | 30/30 | 0/20 | 18/20 | 24/30 | 1/20 | 19/20 | 30/30 | 0/20 | 20/20 |
| 5 | 30/30 | 2/20 | 17/20 | 28/30 | 1/20 | 20/20 | 30/30 | 0/20 | 20/20 |
| 6 | 30/30 | 1/20 | 18/20 | 28/30 | 1/20 | 19/20 | 30/30 | 0/20 | 20/20 |
| 7 | 26/30 | 0/20 | 19/20 | 27/30 | 2/20 | 19/20 | 30/30 | 2/20 | 20/20 |
| 8 | 30/30 | 3/20 | 20/20 | 26/30 | 2/20 | 20/20 | 27/30 | 0/20 | 19/20 |
| 9 | 30/30 | 0/20 | 20/20 | 21/30 | 1/20 | 18/20 | 25/30 | 7/20 | 18/20 |
| 10 | 27/30 | 1/20 | 19/20 | 24/30 | 1/20 | 20/20 | 27/30 | 6/20 | 18/20 |
| 11 | 30/30 | 2/20 | 20/20 | 29/30 | 2/20 | 18/20 | 27/30 | 4/20 | 18/20 |
| 12 | 30/30 | 1/20 | 19/20 | 30/30 | 1/20 | 19/20 | 30/30 | 2/20 | 19/20 |
| Total | 348/360 | 14/240 | 232/240 | 317/360 | 16/240 | 228/240 | 349/360 | 22/240 | 229/240 |
| % Ura+ | 96.7% | 5.8% | 96.7% | 88.1% | 6.7% | 95.0% | 96.9% | 9.2% | 95.4% |
Twelve [PSI+] isolates in each haploid strain were obtained and were either subcloned on YPAD medium or were mated with isogenic WT strain MS173 and the each of diploids formed were subcloned on YPAD medium. Each haploid strain, heterozygous- and homozygous-diploids were replica-plated to −Ura plates to test the stability of [PSI+]. Each prion variant was more stable in its original host (haploids) or homozygous diploids than in the complemented (heterozygous) diploids.