Table 4.
Strain | GFP signals | ||
Diffused, % | Single foci, % | Multiple foci, % | |
WT [pin−] | 100 | 0 | 0 |
WT [PIN+]original | 35.6 | 47.1 | 17.3 |
WT [PIN+]WT | 35.0 | 47.4 | 17.6 |
WT [PIN+]ssb1/2∆ | 34.5 | 42.6 | 22.9 |
WT [PIN+]zuo1∆ | 19.5 | 40.8 | 39.7 |
WT [PIN+]ssz1∆ | 18.9 | 41.9 | 39.2 |
Strains MS317, MS562-MS565 (top to bottom), transformed with plasmids encoding RNQ1−GFP were grown on SD-Leu. The frequencies of cells with different number of aggregates were scored in 100 to 150 cells. Superscripts in the strain column indicate different sources of [PIN+].