Fig. 4.

Split-open cortical collecting ducts from kidney-specific Gpr116 KO do not contain cells that are stimulated by exogenous p16 synthetic agonist peptide. (A) Representative Fura-2 (ΔF340/F380) traces of split-open collecting ducts from WT and KO mice. Traces are mean ± SEM of all ATP-responsive cells in three split-open collecting ducts. (B) Summary data of ΔF340/F380 for all ATP-responsive cells observed in split-open collecting ducts from WT and KO mice. Data are normalized to ΔF340/F380 of WT tubules to ATP. Bars are mean ± SEM. n = 63 WT cells, 37 KO cells. Statistical analysis performed using Kruskal–Wallis test followed by Dunn’s multiple comparisons.