Fig. 6.

Kidney-specific Gpr116 KO mice have alkaline blood and reduced pCO₂ compared to WT littermates. (A–D) Whole-blood samples harvested from facial vein puncture demonstrate increased blood pH from KO mice, a trend toward increased blood HCO₃⁻, and significantly reduced pCO₂. TCO₂ is not statistically different, but shows a trend toward increased values in KO samples (due to increased HCO₃⁻ in those samples). TCO₂ was calculated as the sum of pCO₂ (in mM) and HCO₃⁻. Bars are mean ± SEM. n = 39 WT mice, 30 KO mice. Statistical analysis performed using Mann–Whitney test. *P < 0.05. Blood pH: WT vs. KO *P = 0.002; blood HCO₃⁻: WT vs. KO P = 0.06; pCO₂: WT vs. KO *P = 0.04. TCO₂: WT vs. KO P = 0.06.