FIG 3.
Expression of glpT and ugpC responds to carbon availability. Shown are data for the expression of glpT (A), ugpC (B), and phoB (C) in wild-type S. aureus after 10 h of growth in PFM9 with 5 mM Pi and 5 mM either glycerol or G3P as the sole carbon source. Expression was assessed by measuring fluorescence using the PglpT-yfp, PugpC-yfp, and PphoB-yfp reporter plasmids. Strains were precultured overnight in TSB. *, P < 0.05 for G3P compared to glycerol by an unpaired t test (n = 3); n.s., not significant. Error bars indicate SEM.