FIG 5.
Use of G3P as a phosphate source by S. aureus is PhoB and Pi transporter dependent. Shown are data for endpoint growth after 10 h of wild-type S. aureus and the indicated mutants (Δpst indicates ΔpstSCAB) in PFM9 with 0.5% glucose supplemented with the indicated concentrations of Pi (A and B) or G3P (C to F) as the phosphate source and buffered to pH 7.4 (A and C) or pH 6.4 (B, D, and F). Strains were precultured overnight in a Pi-limiting medium at the permissive pH so that the cells were equivalently phosphate limited at the start of the growth assay. *, P < 0.05 compared to the wild type by two-way ANOVA with Tukey’s multiple-comparison test; #, P < 0.05 for the indicated comparison by two-way ANOVA with Tukey’s multiple-comparison test (n ≥ 3). Error bars indicate SEM.