TABLE 1.
PhoB and Pi transporter dependencies of 58 phosphorus-containing compounds screened for growth of S. aureusa
| Source |
|---|
| Nonphosphate sources |
| Triethyl phosphate |
| Hypophosphite |
| Adenosine-3′,5′-cyclic monophosphate |
| Guanosine-3′,5′-cyclic monophosphate |
| 2-Deoxy-d-glucose-6-phosphate |
| Cytidine-3′-monophosphate |
| Cytidine-3′,5′-cyclic monophosphate |
| Uridine-3′,5′-cyclic monophosphate |
| Phosphonoacetic acid |
| 2-Aminoethyl phosphonic acid |
| Methylene diphosphonic acid |
| Thymidine-3′,5′-cyclic monophosphate |
| Pi transporter-dependent, PhoB-independent phosphate sources |
| AMP |
| Guanosine-2′-monophosphate |
| GMP |
| Guanosine-2′,3′-cyclic monophosphate |
| d-Glucose-6-phosphate |
| CMP |
| Uridine-3′-monophosphate |
| UMP |
| Thymidine-3′-monophosphate |
| dTMP |
| PhoB- and Pi transporter-independent phosphate sources |
| Thiophosphate |
| Dithiophosphate |
| Carbamoyl phosphate |
| Guanosine-3′-monophosphate |
| Phosphoglycolic acid |
| Cysteamine S-phosphate |
| Phospho-l-arginine |
| O-Phospho-d-tyrosine |
| PhoB- and Pi transporter-dependent phosphate sources |
| Pyrophosphate |
| Trimetaphosphate |
| Tripolyphosphate |
| Adenosine-2′-monophosphate |
| Adenosine-3′-monophosphate |
| Adenosine-2′,3′-cyclic monophosphate |
| d,l-α-Glycerol phosphate (G3P) |
| β-Glycerol phosphate |
| d-2-Phosphoglyceric acid |
| d-3-Phosphoclyceric acid |
| Phosphoenolpyruvate |
| d-Glucose-1-phosphate |
| d-Glucosamine-6-phosphate |
| 6-Phosphogluconic acid |
| Cytidine-2′-monophosphate |
| Cytidine-2′,3′-cyclic monophosphate |
| d-Mannose-1-phosphate |
| d-Mannose-6-phosphate |
| O-Phospho-d-serine |
| O-Phospho-l-serine |
| O-Phospho-l-threonine |
| Uridine-2′-monophosphate |
| Uridine-2′,3′-cyclic monophosphate |
| O-Phospho-l-tyrosine |
| Phosphocreatine |
| Phosphorylcholine |
| O-Phosphorylethanolamine |
| Inositol hexaphosphate |
Categorization of phosphate sources was based on the endpoint growth of S. aureus PhoB- and/or Pi transporter-deficient strains normalized to wild-type growth on Pi. Nonphosphate sources were those on which wild-type S. aureus did not reach a terminal OD600 of >0.1. PhoB- and Pi transporter-dependent phosphate sources were those in which ΔphoB mutants reached a terminal OD600 of <70% of that of the wild type at either the permissive or nonpermissive pH. Pi transporter-dependent, PhoB-independent substrates were classified as those on which the growth of the ΔpstSCAB ΔpitA mutant was <70% of that of the wild type at the nonpermissive pH compared to growth at the permissive pH. PhoB- and Pi transporter-independent phosphate sources were those in which all mutants grew to ≥70% of the growth of the wild type at both pHs.