TABLE 3.
Oligonucleotides used in this study
| Name | Sequence (5′→3′)a |
|---|---|
| oDB0071 | CTGAGCTCCTGCAGTAAAGGAGAAAATTTTATGTTTTCTAAAAAAAATGAT |
| oDB0072 | TAGGATCCGGTTAATTAATTTTCTCTACAGCT |
| oCDTcdCmyc3 | TAGGATCCTTATAAATCTTCTTCACTTATTAATTTTTGTTCTAAATCTTCTTCACTTATTAATTTT |
| oCD_SortaseF | GTCTGAGCTCCTGCAGTAAAGGAGAAAATTTTATGTTGAAAAAATTATATAGAATAG |
| oCD_SortaseR | CCCTCGAGAAATCAATCTACCATGAATCAC |
| oTcdCRev | AAACTCGAGAATTAATTTTCTCTACAGCTATCCCTGG |
| CDTcdCC51SF | CAATATATCCTCACCAGCTAGTTCTGAAGACCATGAGGAG |
| CDTcdCC51SR | CTCCTCATGGTCTTCAGAACTAGCTGGTGAGGATATATTG |
| oJC424 | CAATATATCCTCACCAGCTGCTTCTGAAGACCATGAGGAG |
| oJC425 | CTCCTCATGGTCTTCAGAAGCAGCTGGTGAGGATATATTG |
a
The restriction enzyme sites used are underlined.