FIG 5.

Requirement for acidic amino acids in extracellular sensor loop of GraS is dependent on growth conditions. (A) OD₆₀₀ after 12 h of growth in TSB plus 10 μg/ml PmB or TSB (pH 5.5) plus 20 μg/ml PmB. (B) Growth in TSB (pH 5.5) containing 250 μM LA. (C) Growth in TSB (pH 5.5) supplemented with 20 μg/ml PmB and 25 μM palmitoleic acid. Each data point represents the mean ± SE from triplicate cultures. Statistical significance for panel A was measured using one-way ANOVA. ****, P < 0.0001; n.s., not significant.