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Seven DUF1127 proteins are predicted and produced in A. tumefaciens. (A) Characteristics of three SDPs and four LDPs. *, protein annotation was incorrect and adjusted at the N terminus (see Fig. S2B in the supplemental material). (B) Western blot analysis of chromosomally integrated C-terminal 3×FLAG fusions of all seven DUF1127 proteins at different growth phases in YEB medium at 30°C. A heat shock was applied for 20 min at 42°C and cold shock for 20 min at 17°C.
