FIG 10.

Growth phase-dependent differences in the proteomes of WT and ΔΔΔ strains. (A) Crude extracts from different growth phases were separated by SDS-PAGE and stained with Coomassie brilliant blue. During growth phase II, the ΔΔΔ mutant showed an additional protein band between 35 and 40 kDa. The proteins identified in this band by mass spectrometry were PstS (36.2 kDa) and Adh (37.6 kDa). (B) Growth phase-dependent expression of pstS and adh in WT and ΔΔΔ strains was confirmed by Northern blotting. EtBr-stained 16S rRNA served as a loading control.