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. 2020 Sep 23;12(18):18181–18191. doi: 10.18632/aging.103666

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Copyright: © 2020 Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Knockdown of NLRP3 in HAECs. HAECs were transfected with either a scramble sequence (scr), or a short hairpin small interfering RNA for NLRP3 (shNLRP3). (A) All plasmids also carried a red fluorescent protein (RFP), which allowed the transfected cells to be visualized and isolated by flow cytometry based on RFP, shown by representative flow charts. (B) The purified transfected cells were visualized due to RFP in culture. (C, D) NLRP3 levels in purified cells by RT-qPCR (C) and by ELISA (D). (E, F) IL-1β (E) and caspase 1 (F) levels in purified cells by ELISA. *p<0.05. N=5. Scale bars are 20 μm.