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Endothelial cell apoptosis and ROS production are attenuated in HFD-treated endothelia-specific NLRP3 knockout mice. (A, B) CD31+ cells were isolated from the dissociated mouse aortic arch by flow cytometry, shown by representative flow charts (A), and by quantification (B). (C, D) The apoptosis of isolated CD31+ cells were analyzed by FITC Annexin V Apoptosis assay, shown by quantification (C), and by representative flow charts (D). (E) DHE assay for ROS. *p<0.05. N=10.
