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NLRP3/ caspase-1/IL-1β are depleted in endothelial cells in APOEKO/Tie2p-Cre/NLRP3MKO mice. The knockdown of NLRP3/ caspase-1/IL-1β cascades in APOEKO/Tie2p-Cre/NLRP3MKO mice was confirmed on CD31+ endothelial cells isolated from mouse aortic arch. (A, B) NLRP3 levels by RT-qPCR (A) and by ELISA (B). (C, D) IL-1β (C) and caspase 1 (D) levels by ELISA. *p<0.05. NS: non-significant. N=5.
