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. 2020 Jul 23;12(18):18073–18083. doi: 10.18632/aging.103608

Table 1. Sequences of primers, siRNAs, microRNA mimic, and microRNA inhibitor used in this study.

Gene Sequence (5’-3’)
MiR-141-3p Forward: GGTCCTAACACTGTCTGGTAAAGTGG
Reverse: CCAGTGCAGGGTCCGAGGT
U6 Forward: TGCGGGTGCTCGCTTCGGCAGC
Reverse: CCAGTGCAGGGTCCGAGGT
GAPDH Forward: AGACCACAGTCCATGCCATC
Reverse: CAGGGCCCTTTTTCTGAGCC
TNF-α Forward: GACAGCAGAGGACCAGCTAA
Reverse: AGCTGTCATATTTCCCGCTCT
IL-6 Forward: CATCCCATAGCCCAGAGCAT
Reverse: CAGGCTGGCATTTGTGGTTG
RIPK1 siRNA Forward: GAAUGAGGCUUACAACAGTT
Reverse: CUGUUGUAAGCCUCAUUCTT
Si-NC AAUUCUCCGAACGUGUCACGU
MiR-141-3p mimic Forward: UAUUGCACAUUACUAAGUUGCA
Reverse: CAACUUAGUAAUGUGCAAUAUU
Mimic NC UUCUCCGAACGUGUCACUGUU
MiR-141-3p inhibitor UGCAACUUAGUAAUGUGCAAUA
Inhibitor NC CAGUACUUUUGUGUAGUACAA

U6: U6 small nuclear RNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; TNF-α: Tumor necrosis factor alpha; IL-6: Interleukin 6; siRNA: Small interfering RNA; NC: Negative control.