Table 1. Sequences of primers, siRNAs, microRNA mimic, and microRNA inhibitor used in this study.
Gene | Sequence (5’-3’) |
MiR-141-3p | Forward: GGTCCTAACACTGTCTGGTAAAGTGG |
Reverse: CCAGTGCAGGGTCCGAGGT | |
U6 | Forward: TGCGGGTGCTCGCTTCGGCAGC |
Reverse: CCAGTGCAGGGTCCGAGGT | |
GAPDH | Forward: AGACCACAGTCCATGCCATC |
Reverse: CAGGGCCCTTTTTCTGAGCC | |
TNF-α | Forward: GACAGCAGAGGACCAGCTAA |
Reverse: AGCTGTCATATTTCCCGCTCT | |
IL-6 | Forward: CATCCCATAGCCCAGAGCAT |
Reverse: CAGGCTGGCATTTGTGGTTG | |
RIPK1 siRNA | Forward: GAAUGAGGCUUACAACAGTT |
Reverse: CUGUUGUAAGCCUCAUUCTT | |
Si-NC | AAUUCUCCGAACGUGUCACGU |
MiR-141-3p mimic | Forward: UAUUGCACAUUACUAAGUUGCA |
Reverse: CAACUUAGUAAUGUGCAAUAUU | |
Mimic NC | UUCUCCGAACGUGUCACUGUU |
MiR-141-3p inhibitor | UGCAACUUAGUAAUGUGCAAUA |
Inhibitor NC | CAGUACUUUUGUGUAGUACAA |
U6: U6 small nuclear RNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; TNF-α: Tumor necrosis factor alpha; IL-6: Interleukin 6; siRNA: Small interfering RNA; NC: Negative control.