Fig. 6.

Common Epigenetic effect of different ARTs. (a) K-means clustering for the all merged hyper- (up) and hypo-DMRs (down) of the seven comparisons. The hyper- and hypo-DMRs of the seven comparisons were merged if they have 1-bp common region at least, respectively. In green-purple heatmaps, DMRs were classified into twelve clusters by k-means clustering on row scaled average DNA methylation level. With the same row order, row scaled DNA methylation level in each neonatal sample was also shown in cyan-red heatmaps. The number of DMRs in the selected clusters were also shown. (b) For the selected clusters of hyper-DMRs (C21, C22) and hypo-DMRs (C23, C24), all enrichment terms of gene ontology (biological process) with adjusted p-value < 0.05 were grouped and shown (The p-values were attained by the hypergeometric test and adjusted by BH method). (c) Venn diagrams displayed the overlap of the gain H3K4me3 DPs for ICSI-ET versus CTRL, IVF-FET versus CTRL, ICSI-ET versus CTRL, and IVF-FET versus IVF-ET. (d) The chromatin states distribution in each neonatal sample for the selected DMRs and overlapping gain H3K4me3 DPs. The chromatin states were defined in Fig. 3b.