Table 2.

Primer sequences, amplicon lengths and efficiency for qPCR assays

Gene description and purpose	Primer sequence (forward / reverse)	Amplicon length (bp)	qPCRa Efficiency
VvHyd2 PCR	CAAGTATCTGGGCGAAAACC / CATTCTGGGGAAGAGCAAA	509	–
VvHyd2 qPCR	CATCATAGGCGTCATCTTCG / GGACTCTTGCTCTTCCGTGA	118	1.93
VvUGT PCR	TAAATACGTTTATTGAGCTTGA / ACACCGTACCATATGCTTTC	468	–
VvUGT qPCR	TCGCTTCTTGTGGTCCCTTC / CCAATTCTAGCCGTCCGATG	120	1.94
VvBG2 PCR	TCTGGCACCTCTGCTTATCA / CCCTGTTGTGTTTCCTGATACT	644	–
VvBG2 qPCR	TTTGCTTTGGGTGGTTATGA / GCCAACAAGATGTGATGTGC	125	1.93
VvNCED1 qPCR	CCTCTGTCTCACAGCAATGGAACT / ATTACCGGCGATTTGCACTCT	140	1.96

^a The qPCR efficiency was calculated using LinRegPCR software [42]