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. Author manuscript; available in PMC: 2020 Oct 24.
Published in final edited form as: ACS Chem Biol. 2020 Jul 6;15(7):1801–1807. doi: 10.1021/acschembio.0c00369

Figure 3. ENH1 inhibits parasite growth through CDPK1-dependent and -independent pathways.

Figure 3.

(a) Egress assays of wild-type CDPK1G and CDPK1M parasites treated with ENH1 followed by the egress agonist zaprinast. (b) Plaque assays of CDPK1G and CDPK1M parasites treated with ENH1. (c) Selected frames of time-lapse microscopy of CDPK1G and CDPK1M parasites expressing the genetically encoded calcium indicator GCaMP6f with ENH1. Scale bar is 10 μm. Time is indicated as minutes:seconds. (d) Kymographs showing the median fluorescence intensities relative to the initial intensity of 99 parasite vacuoles (rows) 10 minutes following treatment with ENH1.