Figure 4. Thermal profiling in CDPK1^M parasite lysates reveals other potential targets of ENH1.

(a) Thermal profiling strategy in CDPK1^M parasite lysates with 2 mM calcium. Detergent-solubilized parasite lysates were combined with ENH1 or vehicle, heated, and processed analogously to the intact-cell profiling experiment. (b) A side-by-side comparison of the CDPK1 thermal stability profiles obtained from the in-cell (CDPK1^G) and lysate (CDPK1^M) experiment. Circles and triangles denote replicates. (c) A comparison of thermal stability shifts measured in the in-cell (CDPK1^G) and lysate (CDPK1^M) experiment. Proteins exhibiting significant shifts in both experiments are highlighted. (d) A table of proteins exhibiting significant ENH1-dependent changes in thermal stabilization. The direction of alteration to the thermal profile in each experiment is indicated by + (stabilization) or – (destabilization).