Figure 4.

Expression of proangiogenic factors after corneal alkali burn injury. RNA isolated from WT and Nox4 KO corneas 7 days after NaOH treatment. Injury markedly increased the expression of proangiogenic factors: (A) VEGF and (B) TGFβ1 in WT corneas. The upregulation of proangiogenic factors was reduced in the absence of Nox4. Values (mean ± SEM; n = 4) are expressed as a fold change compared with untreated WT control; *P < 0.05 from WT without NaOH treatment; ^#P < 0.05 between WT and Nox4 KO with NaOH treatment. Data were analyzed with one-way ANOVA followed by post hoc Tukey analysis.