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. 2020 Oct 23;10:18193. doi: 10.1038/s41598-020-75328-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analyses. (A) Immunohistochemical images of OCN, COL1a1 and BMP2 expressed in newly-formed bone in defected area (blue arrowheads indicate the expression region of each protein). Integrated optical density (IOD) value measured by image analysis of OCN (B), COL1a1 (C) and BMP2 (D) (*p < 0.05, **p < 0.01, and ***p < 0.001 versus the indicated group). The bone defects in control group were kept empty.