Skip to main content
. 2020 Oct 23;11(10):909. doi: 10.1038/s41419-020-03116-2

Fig. 6. Histone deacetylase 6 (Hdac6) is involved in MRPs-induced TDP-43 co-aggregation.

Fig. 6

A Confirmation of Hdac6 knock-down in N2a cells by the treatment with siRNA (siHdac6). The expression levels relative to the control siRNA treated samples (siCtrl) were shown. B–D Hdac6 suppression specifically prevented MRPs-induced TDP-43 co-aggregation but not RBPs. Representative images are shown in B. Arrowheads indicate co-aggregates of the MRPs and RBPs with TDP-43∆NLS-EGFP. Whereas, asterisks indicate the cells without co-aggregates of the MRPs by Hdac6 suppression. The ratio of the cells with MRPs aggregates (C) and the ratio of MRPs and TDP-43 co-aggregation were substantially reduced, specifically by siHdac6 treatment (D). More than 50 cells in each condition were analyzed for the quantification. All the data are expressed as mean ± SEM (n = 3).