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. 2020 Oct 23;11:5357. doi: 10.1038/s41467-020-19155-4

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© The Author(s) 2020, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The transverse dark-brown line (blue arrow) indicates the normal position of the axis. The yellow arrow indicates the position of the second ectopic axis. Wnt1 induced formation of the second ectopic axis, and successfully inhibiting Wnt resulted in the formation of a single axis. Embryos at the four-cell stage were co-injected in a ventro-vegetal blastomere with 30 pg Wnt1 mRNA (n = 104), 25 pg WT SOST mRNA (n = 106), 25 pg SOST_tr169 mRNA (n = 98), 25 pg SOST_tr177 mRNA (n = 119), 25 pg SOST_tr204 mRNA (n = 104), and 25 pg SOST∆loop2 mRNA (n = 96) (n number of embryos). b The percentage of embryos with a second axis. The error bars represent the standard error of the mean (SEM) from three independent experiments (n = 3) and p value by two-tailed t-test is indicated. The number of injected embryos is described in a. c, d Real-time qPCR analyses of the expression of genes directly targeted by Wnt1, namely siamois (c) and nodal 3.1 (d) from animal cap explants isolated at stage 8 and grown to stage 11. The error bars represent SEMs from three independent experiments (n = 3) and p values by two-tailed t-test are indicated.