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. 2020 Oct 19;15:7979–7993. doi: 10.2147/IJN.S275650

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© 2020 Wu et al.

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Exosomal miR-21-5p regulates SPRY2 by directly targeting the 3ʹ-UTR. ns: no significance; (**) p < 0.01, (***) p < 0.001. (A) The miR-21-5p binding sequence in the 3ʹ-UTR of SPRY2. (B) A luciferase reporter assay was performed to confirm that SPRY2 is the target gene of miR-21-5p. (C) mRNA level of SPRY2 in HUVECs and HSFs after transfection with miR-21-5p mimic or miR-21-5p inhibitor or their negative controls. (D) Quantitative analysis of the wound size recovery rate of HUVECs after transfection with miR-21-5p mimic or si SPRY2 or their negative controls. (E and F) Quantitative analysis of the migration rate of HUVECs and HSFs after transfection with miR-21-5p mimic, si SPRY2 or their negative controls. (G) qRT-PCR analysis of VEGF, HIF-1α, PDGFRα and bFGF after transfection with miR-21-5p mimic, si SPRY2 or their negative controls. (H) Western blotting assay for the protein expression of AKT, p-AKT, Erk1/2, p-Erk1/2, VEGF, HIF-1α, PDGFRα and bFGF after transfection with miR-21-5p mimic, si-SPRY2 or their negative controls.