Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers Used for qRT-PCR
| Gene | Sequence |
|---|---|
| Col1A1 | Forward: 5ʹ-GATCCTGCCGATGTCGCTA-3ʹ |
| Reverse: 5ʹTGTAGGCTACGCTGTTCTTGCA-3ʹ | |
| TGF-β1 | Forward: 5ʹ-TGAGTGGCTGTCTTTTGACG-3ʹ |
| Reverse: 5ʹ-TGGGACTGATCCCATTGATT-3ʹ | |
| α-SMA | Forward: 5ʹ-CGAAGCGCAGAGCAAGAGA −3ʹ |
| Reverse: 5ʹ-CATGTCGTCCCAGTTGGTGAT-3ʹ | |
| Caspase-3 | Forward: 5ʹ-GAGACAGACAGTGGAACTGACGATG-3ʹ |
| Reverse: 5ʹ-GGCGCAAAGTGACTGGATGA-3ʹ | |
| GAPDH | Forward: 5ʹ-GACAACTTTGGCATCGTGGA-3ʹ |
| Reverse: 5ʹ-ATGCAGGGATGATGTTCTGG-3ʹ |
