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. 2020 Oct 24;11(10):913. doi: 10.1038/s41419-020-03110-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Representative immunoblot of Atg7 protein in the cochlea. B Expression of the Atg7 gene was analyzed by RT-PCR. C Atg7 was evident in OHCs and IHCs in the cochlear cryosections. D, E Atg7 puncta were distributed throughout the OHC cell bodies as indicated in the infracuticular, nuclear, and basal pool sections. N = 6 for each group (western blot: N = 3). Scale bar: A–C: 5 μm; D, E: 10 μm.