Fig. 3.

miR-224 represses BTRC-related Wnt/β-catenin pathway. a The position of BTRC in Wnt pathway in KEGG pathway map. β-TrCP is an alias of BTRC. b The protein expression of BTRC, pGSK3β-Ser9, and β-catenin in HCT-116 and DLD-1 cells after 48 h transfection with miR-224 inhibitor and negative control (NC) determined by western blot. GAPDH was used as an endogenous control. c–e The quantification of the protein expression levels of BTRC, pGSK3β-Ser9, and β-catenin, respectively, normalized to negative controls. Western blot bands were quantified by ImageJ and GAPDH was used as an endogenous control. *p < 0.05, **p < 0.01. ***p<0.001