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. 2020 Oct 24;10(11):485. doi: 10.1007/s13205-020-02477-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The positive expression of BTRC is stronger in CRC than in normal tissues. a BTRC transcripts in normal tissues (N) and tumor tissues (T) of READ and COAD from TCGA data. TPM: transcripts per million. n.s. = no significance. b The representative IHC pictures of BTRC protein expression in colon normal tissues and CRC tissues by the Human Protein Atlas. Scale bar is 200 μm (upper) and 100 μm (lower). The single arrowheads point to the positive intensity of BTRC in normal colon tissues, and the double arrowheads point to the positive intensity of BTRC in colon adenocarcinoma. BTRC expression in colon adenocarcinoma was lower than in colon normal tissues. c ImageJ was used to analyze the relative mean gray values of BTRC protein expression in IHC pictures of colon normal tissues (n = 3) and colon adenocarcinoma (n = 3) which were normalized to the corresponding mean gray values of stromal tissues in each IHC picture from the Human Protein Atlas. Relative gray value = (The intensity of BTRC expression in normal colorectal epithelium or colorectal tumor)/The intensity of BTRC expression in colorectal stromal cells. *p < 0.05