Table 1.
List of molecular markers (SSRs and InDels) used in the study along with their map positions and genetic diversity parameters
S. no | Marker name | Chromosome No | Map position (bp)b | Major allele frequency | Allele No | Gene diversity (He) | Landraces (proportion) showing > 1 alleles | PICa | Marker typec | Availabilityd |
---|---|---|---|---|---|---|---|---|---|---|
1 | RM495 | 1 | 2,15,956 | 0.56 | 8 | 0.64 | 0.01 | 0.6 | SSR | GDB |
2 | RM1 | 1 | 46,35,793 | 0.18 | 17 | 0.9 | 0 | 0.89 | SSR | GDB |
3 | RM283 | 1 | 48,85,915 | 0.43 | 9 | 0.68 | 0 | 0.63 | SSR | GDB |
4 | RM259 | 1 | 74,45,627 | 0.22 | 10 | 0.84 | 0 | 0.82 | SSR | GDB |
5 | RM312 | 1 | 91,06,659 | 0.42 | 5 | 0.67 | 0 | 0.62 | SSR | GDB |
6 | RM23 | 1 | 91,06,659 | 0.32 | 6 | 0.77 | 0 | 0.73 | SSR | GDB |
7 | R1M7 | 1 | 1,05,18,002 | 0.98 | 2 | 0.05 | 0.01 | 0.05 | InDel | PBS |
8 | RM229 | 1 | 1,84,07,879 | 0.41 | 8 | 0.72 | 0 | 0.67 | SSR | GDB |
9 | RM5 | 1 | 2,39,71,321 | 0.28 | 10 | 0.8 | 0 | 0.77 | SSR | GDB |
10 | R1M30 | 1 | 2,46,60,853 | 0.7 | 2 | 0.42 | 0.05 | 0.33 | InDel | PBS |
11 | RM237 | 1 | 2,83,39,373 | 0.37 | 9 | 0.76 | 0 | 0.73 | SSR | GDB |
12 | RM212 | 1 | 3,30,53,493 | 0.64 | 6 | 0.53 | 0 | 0.49 | SSR | GDB |
13 | RM1003 | 1 | 3,34,75,908 | 0.52 | 6 | 0.61 | 0 | 0.54 | SSR | GDB |
14 | RM3403 | 1 | 3,49,95,620 | 0.3 | 22 | 0.85 | 0 | 0.84 | SSR | GDB |
15 | R1M47 | 1 | 3,68,28,634 | 0.89 | 2 | 0.2 | 0.01 | 0.18 | InDel | PBS |
16 | RM431 | 1 | 3,88,93,890 | 0.3 | 8 | 0.79 | 0.01 | 0.76 | SSR | GDB |
17 | RM154 | 2 | 10,83,820 | 0.18 | 13 | 0.88 | 0.01 | 0.87 | SSR | GDB |
18 | RM279 | 2 | 28,82,052 | 0.16 | 15 | 0.91 | 0.03 | 0.9 | SSR | GDB |
19 | R2M10 | 2 | 71,07,574 | 0.99 | 2 | 0.01 | 0 | 0.01 | InDel | PBS |
20 | RM53 | 2 | 77,06,705 | 0.28 | 15 | 0.84 | 0 | 0.82 | SSR | GDB |
21 | RM452 | 2 | 95,63,257 | 0.37 | 7 | 0.73 | 0.01 | 0.69 | SSR | GDB |
22 | RM521 | 2 | 1,08,04,792 | 0.49 | 12 | 0.72 | 0 | 0.7 | SSR | GDB |
23 | R2M24 | 2 | 1,12,09,429 | 0.89 | 2 | 0.2 | 0.01 | 0.18 | InDel | PBS |
24 | RM300 | 2 | 1,31,91,380 | 0.37 | 12 | 0.75 | 0.01 | 0.72 | SSR | GDB |
25 | R2M26 | 2 | 1,51,04,418 | 0.84 | 2 | 0.27 | 0.01 | 0.23 | InDel | PBS |
26 | RM29 | 2 | 1,74,84,665 | 0.5 | 9 | 0.67 | 0 | 0.62 | SSR | GDB |
27 | R2M37 | 2 | 2,35,63,428 | 0.51 | 2 | 0.5 | 0.05 | 0.37 | InDel | PBS |
28 | RM263 | 2 | 2,58,65,334 | 0.4 | 13 | 0.8 | 0 | 0.78 | SSR | GDB |
29 | RM221 | 2 | 2,76,09,877 | 0.23 | 11 | 0.86 | 0 | 0.84 | SSR | GDB |
30 | R2M50 | 2 | 3,04,06,330 | 0.88 | 2 | 0.21 | 0.01 | 0.19 | InDel | PBS |
31 | RM60 | 3 | 1,05,852 | 0.37 | 11 | 0.73 | 0 | 0.69 | SSR | GDB |
32 | RM81 | 3 | 19,45,837 | 0.29 | 9 | 0.78 | 0 | 0.75 | SSR | GDB |
33 | RM489 | 3 | 43,33,680 | 0.48 | 14 | 0.73 | 0 | 0.71 | SSR | GDB |
34 | R3M10 | 3 | 60,44,614 | 0.99 | 2 | 0.02 | 0 | 0.02 | InDel | PBS |
35 | RM7 | 3 | 1,24,07,382 | 0.39 | 8 | 0.72 | 0 | 0.68 | SSR | GDB |
36 | RM338 | 3 | 1,32,21,482 | 0.68 | 7 | 0.5 | 0 | 0.46 | SSR | GDB |
37 | R3M23 | 3 | 1,56,78,406 | 0.89 | 2 | 0.2 | 0.01 | 0.18 | InDel | PBS |
38 | R3M30 | 3 | 1,98,88,471 | 0.96 | 2 | 0.07 | 0 | 0.07 | InDel | PBS |
39 | RM6832 | 3 | 2,24,00,917 | 0.66 | 11 | 0.53 | 0 | 0.5 | SSR | GDB |
40 | R3M37 | 3 | 2,64,74,984 | 0.63 | 3 | 0.51 | 0.03 | 0.44 | InDel | PBS |
41 | RM55 | 3 | 2,82,31,692 | 0.39 | 9 | 0.75 | 0 | 0.72 | SSR | GDB |
42 | R3M53 | 3 | 3,24,85,490 | 0.64 | 2 | 0.46 | 0.02 | 0.36 | InDel | PBS |
43 | RM514 | 3 | 3,52,81,232 | 0.27 | 12 | 0.84 | 0 | 0.83 | SSR | GDB |
44 | R4M13 | 4 | 81,15,751 | 0.89 | 2 | 0.2 | 0 | 0.18 | InDel | PBS |
45 | RM307 | 4 | 86,10,617 | 0.25 | 12 | 0.84 | 0 | 0.82 | SSR | GDB |
46 | R4M17 | 4 | 1,16,36,507 | 0.54 | 2 | 0.5 | 0.04 | 0.37 | InDel | PBS |
47 | RM16623 | 4 | 1,24,31,839 | 0.09 | 30 | 0.95 | 0 | 0.95 | SSR | GDB |
48 | R4M30 | 4 | 1,81,72,382 | 0.56 | 2 | 0.49 | 0.01 | 0.37 | InDel | PBS |
49 | RM471 | 4 | 1,88,24,746 | 0.28 | 10 | 0.79 | 0 | 0.75 | SSR | GDB |
50 | R4M43 | 4 | 2,45,94,268 | 0.87 | 2 | 0.23 | 0 | 0.2 | InDel | PBS |
51 | RM1388 | 4 | 2,50,35,173 | 0.09 | 28 | 0.95 | 0 | 0.95 | SSR | GDB |
52 | RM241 | 4 | 2,68,57,374 | 0.16 | 19 | 0.92 | 0.01 | 0.91 | SSR | GDB |
53 | R4M50 | 4 | 2,89,89,250 | 0.74 | 2 | 0.39 | 0.02 | 0.31 | InDel | PBS |
54 | RM17467 | 4 | 3,06,74,329 | 0.39 | 24 | 0.8 | 0.04 | 0.79 | SSR | GDB |
55 | RM124 | 4 | 3,47,39,636 | 0.49 | 5 | 0.64 | 0.01 | 0.57 | SSR | GDB |
56 | RM507 | 5 | 1,02,742 | 0.39 | 6 | 0.73 | 0 | 0.69 | SSR | GDB |
57 | RM413 | 5 | 22,12,736 | 0.28 | 13 | 0.86 | 0 | 0.84 | SSR | GDB |
58 | RM3322 | 5 | 42,62,731 | 0.27 | 14 | 0.83 | 0 | 0.81 | SSR | GDB |
59 | R5M13 | 5 | 59,50,163 | 0.87 | 2 | 0.22 | 0 | 0.2 | InDel | PBS |
60 | R5M30 | 5 | 2,14,47,905 | 0.64 | 2 | 0.46 | 0.01 | 0.35 | InDel | PBS |
61 | RM178 | 5 | 2,51,01,829 | 0.55 | 6 | 0.64 | 0 | 0.6 | SSR | GDB |
62 | RM87 | 5 | 2,68,48,154 | 0.59 | 7 | 0.61 | 0 | 0.58 | SSR | GDB |
63 | RM161 | 5 | 2,68,48,154 | 0.5 | 8 | 0.68 | 0.01 | 0.64 | SSR | GDB |
64 | RM334 | 5 | 2,73,42,022 | 0.15 | 13 | 0.89 | 0 | 0.88 | SSR | GDB |
65 | RM133 | 6 | 4,41,616 | 0.75 | 4 | 0.41 | 0 | 0.38 | SSR | GDB |
66 | RM510 | 6 | 28,31,443 | 0.61 | 5 | 0.56 | 0 | 0.5 | SSR | GDB |
67 | RM585 | 6 | 37,22,966 | 0.43 | 9 | 0.76 | 0.01 | 0.74 | SSR | GDB |
68 | RM217 | 6 | 42,12,289 | 0.14 | 21 | 0.92 | 0.01 | 0.91 | SSR | GDB |
69 | RM253 | 6 | 54,25,408 | 0.23 | 15 | 0.88 | 0 | 0.87 | SSR | GDB |
70 | RM2126 | 6 | 59,05,238 | 0.28 | 15 | 0.8 | 0 | 0.77 | SSR | GDB |
71 | RM7488 | 6 | 69,98,987 | 0.27 | 19 | 0.84 | 0.01 | 0.82 | SSR | GDB |
72 | R6M14 | 6 | 74,36,963 | 0.68 | 2 | 0.43 | 0.01 | 0.34 | InDel | PBS |
73 | RM3330 | 6 | 1,10,64,125 | 0.22 | 10 | 0.87 | 0 | 0.86 | SSR | GDB |
74 | RM7434 | 6 | 2,39,34,663 | 0.37 | 7 | 0.71 | 0 | 0.66 | SSR | GDB |
75 | RM7434 | 6 | 2,39,34,663 | 0.25 | 8 | 0.79 | 0.01 | 0.76 | SSR | GDB |
76 | RM162 | 6 | 2,40,35,491 | 0.41 | 8 | 0.71 | 0.01 | 0.66 | SSR | GDB |
77 | R6M44 | 6 | 2,57,35,724 | 0.89 | 2 | 0.2 | 0.01 | 0.18 | InDel | PBS |
78 | RM454 | 6 | 2,85,32,453 | 0.44 | 5 | 0.65 | 0 | 0.58 | SSR | GDB |
79 | RM481 | 7 | 57,35,196 | 0.26 | 16 | 0.88 | 0.04 | 0.87 | SSR | GDB |
80 | R7M7 | 7 | 65,81,196 | 0.76 | 2 | 0.36 | 0 | 0.3 | InDel | PBS |
81 | RM501 | 7 | 67,79,215 | 0.57 | 14 | 0.61 | 0 | 0.56 | SSR | GDB |
82 | RM125 | 7 | 67,79,215 | 0.46 | 7 | 0.68 | 0 | 0.64 | SSR | GDB |
83 | R7M20 | 7 | 1,12,79,013 | 0.5 | 2 | 0.5 | 0.01 | 0.38 | InDel | PBS |
84 | RM418 | 7 | 1,81,32,231 | 0.37 | 14 | 0.79 | 0 | 0.77 | SSR | GDB |
85 | RM11 | 7 | 1,92,56,914 | 0.38 | 9 | 0.75 | 0 | 0.71 | SSR | GDB |
86 | RM455 | 7 | 2,23,50,593 | 0.65 | 3 | 0.48 | 0 | 0.39 | SSR | GDB |
87 | R7M37 | 7 | 2,36,24,859 | 0.99 | 2 | 0.03 | 0.01 | 0.03 | InDel | PBS |
88 | RM505 | 7 | 2,51,01,758 | 0.42 | 4 | 0.65 | 0 | 0.58 | SSR | GDB |
89 | RM234 | 7 | 2,54,72,688 | 0.52 | 10 | 0.66 | 0 | 0.62 | SSR | GDB |
90 | RM118 | 7 | 2,93,39,845 | 0.36 | 7 | 0.73 | 0 | 0.69 | SSR | GDB |
91 | RM408 | 8 | 1,25,275 | 0.42 | 6 | 0.67 | 0 | 0.61 | SSR | GDB |
92 | RM407 | 8 | 5,22,394 | 0.45 | 12 | 0.74 | 0 | 0.71 | SSR | GDB |
93 | RM152 | 8 | 6,82,963 | 0.36 | 8 | 0.78 | 0 | 0.75 | SSR | GDB |
94 | RM22254 | 8 | 6,91,385 | 0.44 | 22 | 0.69 | 0 | 0.64 | SSR | GDB |
95 | RM44 | 8 | 21,14,843 | 0.45 | 9 | 0.67 | 0 | 0.62 | SSR | GDB |
96 | RM25 | 8 | 21,14,843 | 0.38 | 9 | 0.78 | 0 | 0.75 | SSR | GDB |
97 | RM1148 | 8 | 37,39,008 | 0.39 | 8 | 0.74 | 0 | 0.7 | SSR | GDB |
98 | RM6999 | 8 | 39,84,260 | 0.14 | 19 | 0.91 | 0 | 0.91 | SSR | GDB |
99 | RM3481 | 8 | 91,35,139 | 0.22 | 12 | 0.88 | 0 | 0.86 | SSR | GDB |
100 | RM3395 | 8 | 1,02,93,664 | 0.58 | 6 | 0.59 | 0 | 0.54 | SSR | GDB |
101 | R8M23 | 8 | 1,34,82,749 | 0.95 | 2 | 0.2 | 0 | 0.01 | InDel | PBS |
102 | R8M33 | 8 | 2,07,97,429 | 0.65 | 2 | 0.45 | 0.01 | 0.35 | InDel | PBS |
103 | RM284 | 8 | 2,11,42,348 | 0.62 | 5 | 0.57 | 0 | 0.53 | SSR | GDB |
104 | RM433 | 8 | 2,55,90,576 | 0.29 | 11 | 0.8 | 0 | 0.77 | SSR | GDB |
105 | RM447 | 8 | 2,65,46,992 | 0.41 | 10 | 0.66 | 0 | 0.6 | SSR | GDB |
106 | RM316 | 9 | 12,71,123 | 0.32 | 11 | 0.82 | 0 | 0.8 | SSR | GDB |
107 | R9M10 | 9 | 45,15,585 | 0.74 | 2 | 0.38 | 0 | 0.31 | InDel | PBS |
108 | R9M20 | 9 | 93,78,728 | 0.89 | 2 | 0.2 | 0.01 | 0.18 | InDel | PBS |
109 | RM105 | 9 | 1,18,07,446 | 0.58 | 5 | 0.58 | 0 | 0.53 | SSR | GDB |
110 | RM6839 | 9 | 1,45,66,026 | 0.7 | 5 | 0.46 | 0 | 0.42 | SSR | GDB |
111 | R9M30 | 9 | 1,46,47,925 | 0.84 | 2 | 0.27 | 0 | 0.23 | InDel | PBS |
112 | RM257 | 9 | 1,77,19,660 | 0.21 | 12 | 0.85 | 0.01 | 0.83 | SSR | GDB |
113 | R9M42 | 9 | 1,91,15,701 | 0.99 | 2 | 0.02 | 0 | 0.02 | InDel | PBS |
114 | RM215 | 9 | 2,11,89,110 | 0.35 | 7 | 0.75 | 0 | 0.71 | SSR | GDB |
115 | RM205 | 9 | 2,27,20,624 | 0.39 | 18 | 0.77 | 0.01 | 0.74 | SSR | GDB |
116 | RM474 | 10 | 18,18,800 | 0.42 | 19 | 0.78 | 0 | 0.77 | SSR | GDB |
117 | R10M10 | 10 | 52,07,502 | 0.52 | 2 | 0.5 | 0.06 | 0.37 | InDel | PBS |
118 | RM271 | 10 | 53,52,766 | 0.32 | 10 | 0.81 | 0 | 0.79 | SSR | GDB |
119 | R10M17 | 10 | 90,04,292 | 0.97 | 2 | 0.06 | 0 | 0.06 | InDel | PBS |
120 | RM184 | 10 | 1,63,58,895 | 0.32 | 7 | 0.75 | 0 | 0.7 | SSR | GDB |
121 | R10M30 | 10 | 1,71,91,366 | 0.54 | 2 | 0.5 | 0.04 | 0.37 | InDel | PBS |
122 | RM171 | 10 | 1,90,48,795 | 0.3 | 12 | 0.81 | 0 | 0.78 | SSR | GDB |
123 | R10M40 | 10 | 1,96,17,284 | 0.15 | 2 | 0.1 | 0 | 0.01 | InDel | PBS |
124 | RM484 | 10 | 2,10,66,729 | 0.46 | 5 | 0.61 | 0 | 0.53 | SSR | GDB |
125 | RM228 | 10 | 2,22,43,157 | 0.33 | 21 | 0.85 | 0.01 | 0.84 | SSR | GDB |
126 | RM591 | 10 | 2,28,99,617 | 0.17 | 27 | 0.91 | 0 | 0.9 | SSR | GDB |
127 | RM590 | 10 | 2,30,43,156 | 0.33 | 7 | 0.78 | 0 | 0.75 | SSR | GDB |
128 | RM26063 | 11 | 22,56,991 | 0.18 | 20 | 0.9 | 0.01 | 0.89 | SSR | GDB |
129 | RM552 | 11 | 48,43,013 | 0.12 | 17 | 0.91 | 0 | 0.91 | SSR | GDB |
130 | RM536 | 11 | 89,85,132 | 0.27 | 9 | 0.82 | 0 | 0.8 | SSR | GDB |
131 | RM287 | 11 | 1,67,67,319 | 0.29 | 9 | 0.82 | 0 | 0.8 | SSR | GDB |
132 | RM26771 | 11 | 1,73,59,392 | 0.41 | 18 | 0.75 | 0.01 | 0.72 | SSR | GDB |
133 | RM21 | 11 | 1,83,18,826 | 0.2 | 16 | 0.88 | 0 | 0.87 | SSR | GDB |
134 | R11M23 | 11 | 2,01,48,709 | 0.89 | 2 | 0.2 | 0 | 0.18 | InDel | PBS |
135 | RM26981 | 11 | 2,16,89,935 | 0.09 | 38 | 0.96 | 0 | 0.96 | SSR | GDB |
136 | R11M40 | 11 | 2,48,11,386 | 0.71 | 2 | 0.41 | 0.04 | 0.33 | InDel | PBS |
137 | RM144 | 11 | 2,82,81,693 | 0.31 | 13 | 0.84 | 0 | 0.83 | SSR | GDB |
138 | RiceSSR PODIP | 12 | 6,23,664 | 0.26 | 6 | 0.81 | 0 | 0.78 | SSR | MD |
139 | RM19 | 12 | 31,85,384 | 0.23 | 11 | 0.86 | 0 | 0.85 | SSR | GDB |
140 | RM27601 | 12 | 35,53,566 | 0.48 | 16 | 0.71 | 0 | 0.68 | SSR | GDB |
141 | R12M10 | 12 | 42,89,075 | 0.99 | 2 | 0.02 | 0.02 | 0.02 | InDel | PBS |
142 | RM277 | 12 | 88,26,555 | 0.67 | 6 | 0.5 | 0 | 0.45 | SSR | GDB |
143 | RM7102 | 12 | 1,32,11,325 | 0.38 | 8 | 0.72 | 0.01 | 0.68 | SSR | GDB |
144 | R12M27 | 12 | 1,73,79,856 | 0.77 | 2 | 0.35 | 0 | 0.29 | InDel | PBS |
145 | RM2972 | 12 | 1,91,38,384 | 0.45 | 7 | 0.71 | 0 | 0.67 | SSR | GDB |
146 | RM519 | 12 | 1,99,03,791 | 0.36 | 7 | 0.78 | 0 | 0.75 | SSR | GDB |
147 | R12M33 | 12 | 2,00,06,587 | 0.55 | 2 | 0.5 | 0 | 0.37 | InDel | PBS |
148 | R12M43 | 12 | 2,61,02,497 | 0.89 | 2 | 0.19 | 0.01 | 0.17 | InDel | PBS |
Mean | 0.49 | 8.75 | 0.62 | 0.01 | 0.58 |
aPolymorphic Information Content
bPhysical locations of SSR markers were taken from Gramene: a genomics and genetics resource for rice marker database’ (https://www.gramene.org/markers/microsat/) whereas physical locations of InDel markers were accessed from MSU Rice Genome Annotation Project (https://rice.plantbiology.msu.edu/analyses_search_blast.shtml) based on the BAC accession ID given in the published study of Shen et al. (2004)
cSSR: Simple Sequence Repeats, InDel: Insertion Deletion,
dGDB: adopted from ‘Gramene: a genomics and genetics resource for rice marker database’ (https://www.gramene.org/markers/microsat/), PBS: adopted from the published study of Shen et al. (2004), MD: manually designed for this study