FIG.4. NF200 density is reduced following spinal cord injury but not altered by red-light treatment. (A) The schematic representation of the spinal cord illustrates the dorsal, intermediate, and ventral regions of interest for analysis (enclosed by dashed lines, 0.1 mm²). Approximate location of injury is indicated (purple shaded area). (B) Example images of NF200 (green) positive labeling from spinal-cord injured sham- and light-treated groups ipsilateral to the injury at dorsal level at 7 dpi. (C,D) Quantification of NF200⁺ labeling expressed as positive particle density within the region of interest, in the dorsal region of the spinal cord, contralateral (C) and ipsilateral (D) to the injury of sham- and light-treated groups. (E,F) NF200⁺ particle density in the intermediate regions of interest contralateral (E) and ipsilateral (F) to the injury. (G,H) NF200⁺ particle density in the ventral regions of interest contralateral (G) and ipsilateral (H) to the injury. Data for control animals are shown (solid green, mean; dotted green, SEM). All other data expressed as mean ± SEM; n values indicated for each time-point (legend). See text for statistical comparisons. dpi, days post-injury; SCI, spinal-cord injured animals without red-light treatment; SCI+670, spinal-cord injured animals with red-light treatment; SEM, standard error of the mean. Color image is available online.