FIG. 6.

Spinal-cord injury induced astrocyte activation is reduced following red-light treatment. (A) The schematic representation of the spinal cord illustrates the dorsal, lateral, and ventral regions of interest for analysis (enclosed by dashed lines, area of each box: 0.1 mm²). Approximate location of injury is indicated by the purple shaded area. (B) Example images are shown of positive GFAP labeling (green) from untreated and light-treated groups ipsilateral to the injury at the dorsal region at 3 dpi. (C,D) Quantification of GFAP⁺ labeling, expressed as the percentage area of positive label above threshold within the dorsal regions of interest contralateral (C) and ipsilateral (D) to the injury of untreated and light-treated groups. (E,F) GFAP⁺ label in the lateral regions of interest contralateral (E) and ipsilateral (F) to the injury. (G,H) GFAP⁺ label in the ventral regions of interest contralateral (G) and ipsilateral (H) to the injury. For each time-point n values are indicated (legend). Statistical comparisons between SCI and SCI+670 groups across all time-points and regions (black bracket) and across the time-points at different region (black line) are indicated. Dotted line indicates significance across both sides. Data are expressed as mean ± SEM; *p < 0.05, **p < 0.01 (LMER). GFAP, glial fibrillary acidic protein; LMER, linear mixed-effects models; SCI, spinal-cord injured animals without red-light treatment; SCI+670, spinal-cord injured animals with red-light treatment; SEM, standard error of the mean. Color image is available online.