FIG. 8.

IL-1β producing microglia/macrophage population is not affected by red-light treatment following T10 hemi-contusion. (A) The schematic representation of the spinal cord illustrates the dorsal, lateral, and ventral regions of interest for analysis (enclosed by dashed lines, area of each box: 0.1 mm²). Approximate location of injury is indicated by the purple shaded area. (B) Example images shown of IL-1β (red), IBA1 (green), and DAPI (blue) triple-positive cells from spinal-cord injured untreated and light-treated groups at the dorsal level, ipsilateral to the injury at 7 dpi. (C,D) Quantification of IL-1β⁺IBA1⁺DAPI⁺ cells, expressed as triple-positive cell density within the region of interest, are shown for the dorsal region of the spinal cord, contralateral (C) and ipsilateral (D) to the injury of untreated and light-treated groups. (E,F) IL-1β⁺IBA1⁺DAPI⁺ cell density in the lateral regions of interest contralateral (E) and ipsilateral (F) to the injury. (G,H) IL-1β⁺IBA1⁺DAPI⁺ cell density in the ventral regions of interest contralateral (G) and ipsilateral (H) to the injury. For each time-point n values are indicated (legend) . Data are expressed as mean ± SEM. dpi, day post-injury; IL-1β, interleukin 1 beta; SCI, spinal-cord injured animals without red-light treatment; SCI+670, spinal-cord injured animals with red-light treatment; SEM, standard error of the mean. Color image is available online.