Fig. 1.

Identification of MHY2256 as a novel sirtuin (SIRT) inhibitor. (A) Chemical structure of MHY2256. (B) HCT116 cells were treated with various concentrations of MHY2256 and in vitro SIRT1 deacetylase activity was measured using Fluor-de-Lys fluorescent assays. Data are shown as the mean ± SD of three independent experiments. *p<0.05, **p<0.01 and ***p<0.001 vs. vehicle-treated cells. (C) HCT116 cells were treated with MHY2256 for 24 h, and cell lysates were immunoblotted with SIRT1, SIRT2, Ac-forkhead box O1 (FoxO1), or FoxO1 antibodies. Representative results from three independent experiments are shown.