Fig. 3.

Effects of MHY2256 on cell cycle and cell cycle regulatory proteins in HCT116 cells. (A) Cells were treated for 24 h with the indicated concentrations of MHY2256, stained with propidium iodide (PI), and then flow cytometry was used by analyze their distribution in each phase of the cell cycle. Representative results from three independent experiments are shown. (B) Data are shown as the mean ± SD of three independent experiments. Significance was determined using the Student’s t-test (*p<0.05, and **p<0.01 vs. vehicle-treated cells). (C) To detect the protein expression levels of cell cycle regulators, cells were incubated with MHY2256 for 24 h and then cellular proteins were separated and immunoblotted with indicated antibodies. Representative results from three independent experiments are shown. β-actin was used as an internal control.