Fig. 4.

Role of glycogen synthase kinase-3β (GSK3β) in MHY2256-induced G1 arrest of HCT116 cells. (A) Cells were treated with various concentration of MHY2256 for 24 h, and phosphorylated GSK3β (p-GSK3β) and GSK3β were analyzed using western blot analysis. (B) Cells were incubated with 10 µM MHY2256 for 24 h after pretreatment of 10 mM lithium chloride (LiCl) for 1 h and then cell cycle distribution was assessed using flow cytometry. The percentage of each cell population is shown as the mean ± SD of three independent experiments (**p<0.01, ***p<0.001, vs. vehicle-treated cells and ^##p<0.01, vs. MHY2256-treated cells). (C) Total cell lysates were prepared and immunoblotted with G1 transition-related antibodies. β-actin was used as an internal control.