Fig. 3.

Effect of selonsertib on ASK1/MAPK signaling and expression of fibrogenic proteins in HSCs. (A) HSCs were treated with selonsertib at various concentrations (10-50 μM) for 24 or 48 h. Western blotting for ASK1/MAPKs and ECM-related signals was performed with cell lysates obtained from selonsertib-treated cells. (B) Immunofluorescence staining to examine the effect of selonsertib on the expression of p-ASK1. The images are presented at 400× magnification. Data are represented as the mean ± SD (n=4). *p<0.05 and **p<0.01 versus the control.