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Analytical sensitivity for the N1/N2/RP triplex RT-qPCR assay.
| Viral load (copies/mL) | N1 replicates | N1 sensitivity | N2 replicates | N2 sensitivity |
|---|---|---|---|---|
| 2000 | 5/5 | 100% | 5/5 | 100% |
| 1500 | 5/5 | 100% | 5/5 | 100% |
| 1000a | 5/5 | 100% | 5/5 | 100% |
| 500 | 4/5 | 80% | 4/5 | 80% |
Limit of detection or lower viral load where sensitivity keeps 100%.
