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. 2020 Oct 20;12:10397–10409. doi: 10.2147/CMAR.S244374

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© 2020 Wu et al.

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LPS induces GSDMD-N activation and membrane translocation in HT29 cells. (A). Representative immunoblots of total human GSDMD expression and GSDMD-N expression at the surface. Using GAPDH and alpha Na+/K+ATPase to respectively standardize the expression of the total and surface. As the relative intensity about GSDMD and GSDMD-N, quantification of the expression of total and surface is shown in each fraction. Results shown are mean ± SD. n = 3 in each group; *P < 0.05; ***P < 0.001; ****P < 0.0001 compared with control group. (B). Pyroptosis can be induced by LPS. Staining The HT29 cells with PI and analyzing the HT29 cells under a microscope.