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. 2020 Oct 20;14:4363–4376. doi: 10.2147/DDDT.S270124

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© 2020 Song et al.

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PI3K signaling pathway inhibitors promote apoptosis induced by ISL in HCC cells. (A) The related protein levels of PI3K, p-PI3K, Akt, p-Akt, mTOR and p-mTOR were determined by Western blot. Cells were incubated to ISL (25μM) in combination with or without 10 μM LY294002 for 24 h. (B) Cell viability was analyzed by MTT assay after cells were treated with the indicated concentration of ISL with or without LY294002 for 24 h. The data were represented as mean ± standard error of mean (**P<0.01, ***P<0.001 VS ISL group). (C) Cell apoptosis was analyzed by flow cytometry. The data are presented as mean ± standard error of the mean (n=3), **P<0.01 vs the LY294002-untreated group. (D) Western blot analysis of LC3-I/II, Cleaved-PARP, Cleaved-Caspase3, Bcl-2, and Bax in human hepatocellular carcinoma (HCC) cells. *P < 0.05, **P < 0.01, and ***P < 0.001 versus the ISL group.